WebOct 3, 2016 · Cry11Aa binds to A. aegypti alkaline phosphatase (ALP) with high affinity (Fernandez et al. 2006), and an ALP has been reported to regulate Cry11Ba toxicity in A. … WebApr 15, 2024 · Cry11Aa: residues 381, 422, 443, 455, 457, 460, 469, 473, 484, 487, 498 in SEQ ID NO:11. In addition, the polypeptide of this invention further includes a fragment of a modified Cry protein (e.g., a modified Cry3Aa protein) generally corresponding to its domain II, such as the 389-471 fragment of SEQ ID NO:2, which the present inventors have ...
Construction and characterization of the interdomain
WebMay 13, 2024 · Endotoxins, including Cry4Aa, Cry4Ba, Cry10Aa, Cry11Aa, and Cyt1Aa, are toxic to mosquitoes. Insect eradication by Cry toxin relies primarily on the interaction of cry toxins with key toxin receptors, such as aminopeptidase (APN), alkaline phosphatase (ALP), cadherin (CAD), and ATP-binding cassette transporters. WebNov 1, 2024 · We coated Cry11Aa proteins with Mg(OH) 2 nanoparticles (MHNPs), and then assessed the effects of MHNPs on bioactivity and anti-ultraviolet ability of the Cry11Aa proteins. Our results indicated that MHNPs, like "coating clothes", could effectively protect the Cry protein and enhance the insecticidal bioactivity after UV radiation (the ... doubletree by hilton cheltenham spa breaks
(PDF) Bacillus thuringiensis Cyt Proteins as Enablers of Activity of ...
WebDec 1, 2013 · Cry11Aa toxin binds AaeAPN2 with high affinity, and we also identified a toxin binding region in this APN. Here this cloned APN is referred to as AaeAPN2, which has … WebBinding and pore-forming activity of Cry11Aa, which is the most toxic protein against mosquitoes, and Cyt1Aa to brush border membrane vesicles (BBMVs) of T. paludosa were analyzed. Solubilization of Cry11Aa resulted in two fragments, with apparent molecular masses of 32 and 36 kDa. WebNov 11, 2009 · Cry11Aa of Bacillus thuringiensis subsp. israelensis is the most active toxin to Aedes aegypti in this strain. We previously reported that, in addition to a 65 kDa GPI (glycosylphosphatidylinositol)-anchored ALP (alkaline phosphatase), the toxin also binds a 250 kDa membrane protein. doubletree by hilton cheltenham email