High-throughput method

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August undefined, 2024

WebJul 28, 2012 · We developed a high throughput DNA isolation method by combining a high yield CTAB extraction method with an improved cleanup procedure based on MagAttract kit. The method yielded large quantity and high quality DNA from both lyophilized sorghum leaves and dry seeds. WebSep 24, 2024 · We have developed a simple 96-well plate-based high-throughput DNA extraction method that is applicable to many plant species. The method involves a simple incubation of plant tissue samples in a DNA extraction buffer followed by a neutralization step. With the addition of a modified PCR buffer, the extracted DNA enabled the robust ...

High-throughput cultivation and identification of bacteria ... - Nature

WebHigh-throughput flow cytometry is an attractive platform for the analysis of adoptive cellular therapies such as chimeric antigen receptor T cell therapy (CAR-T) because it allows for the concurrent measurement of T cell-dependent cellular cytotoxicity (TDCC) and the functional characterization of engineered T cells with respect to percentage of CAR transduction, T … Web7.1.2.2 High-throughput computing. High-throughput computing (HTC) is the use of distributed computing facilities for applications requiring large computing power over a … comma before since or not

Robust and high-throughput lipidomic quantitation of human …

WebMar 8, 2012 · BIPES, a cost-effective high-throughput method for assessing microbial diversity. ISME J 5: 741–749. Article CAS Google Scholar Download references. Acknowledgements. We wish to thank the ... WebNov 6, 2024 · Here we describe a strategy for ultra high-throughput joint analysis of transcriptome and accessible chromatin that could be used to study millions of individual … WebJul 31, 2024 · In conclusion, our platform provides a high-throughput method to interrogate a wide variety of protein-DNA interactions. The utilities of our DocMF platform can be extended to other applications, such as on-chip identification of off-target sites for a CRISPR-Cas system, single-stranded DNA cleavage sites, or TF binding motifs. comma before somehow at end of sentence

Development of a high-throughput method to screen novel …

What does high-throughput mean? - Definitions.net

WebApr 14, 2024 · Accurately and rapidly counting the number of maize tassels is critical for maize breeding, management, and monitoring the growth stage of maize plants. With the advent of high-throughput phenotyping platforms and the availability of large-scale datasets, there is a pressing need to automate this task for genotype and phenotype … WebHigh throughput cell biology has a definite focus on the cell, and methods accessing the cell such as imaging, gene expression microarrays, or genome wide screening. The basic idea is to take methods normally performed on their own and do a very large number of them without impacting their quality [7] comma before speech marks ukWebDirect infusion of lipid extracts into the ion source of a mass spectrometer is a well-established method for lipid analysis. In most cases, nanofluidic devices are used for sample introduction. ... based on sample infusion from a chromatographic pump can offer a s … Robust and high-throughput lipidomic quantitation of human blood samples ... comma before or after the s

"WebJan 1, 2004 · The DNA microarray technology, the most commonly used method for high-throughput gene expression profiling, has been touted as an integrative tool that provides insights into regulatory pathways. However, the physiology community has been slow in acceptance of these techniques because of early failure in generating useful data and the … " - High-throughput method

High-throughput method

WebJan 21, 2024 · The new method, called single-cell expression-based variant impact phenotyping (sc-eVIP), builds on Perturb-seq—an approach developed in 2016 by Regev … WebJan 20, 2024 · New high-throughput method greatly expands view of how mutations impact cells. Broad scientists have developed a new approach for studying the functional effects …

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WebSep 21, 2024 · The approach is based on a novel high-throughput method inspired by other known approaches such as thin film PVD (physical vapor deposition) sample generation for functional materials or diffusion couples for structural materials [4,5,6,7]. Instead of applying classical measurement methods on macroscopic samples, the typical characterization ... WebJul 13, 2024 · Hybridisation-based targeted enrichment is a widely used and well-established technique in high-throughput second-generation short-read sequencing. Despite the high …

WebMar 9, 2024 · High-throughput single-cell RNA-seq methods assign limited unique molecular identifier (UMI) counts as gene expression values to single cells from shallow sequence reads and detect limited gene counts. We thus developed a high-throughput single-cell RNA-seq method, Quartz-Seq2, to overcome these issues. Our improvements … WebApr 10, 2024 · High-throughput screening (HTS) is a drug discovery process that allows automated testing of large numbers of chemical and/or biological compounds for a …

WebAug 26, 2024 · A BRET-based approach to target engagement and assessment of relative intracellular availability. (a) Schematic representation of a BRET-based biosensor for E3 ligase engagement using BRET with NanoLuc (NLuc).Each complex is comprised of an NLuc-tagged target E3 ligase in dynamic equilibrium with a fluorescent reporter (tracer) … WebSep 11, 2015 · The Gauss-peak spectra (GPS) method represents individual pigment spectra as weighted sums of Gaussian functions, and uses these to model absorbance spectra of phytoplankton pigment mixtures. We here present several improvements for this type of methodology, including adaptation to plate reader technology and efficient model fitting …

WebApr 27, 2024 · In addition, high-throughput techniques can be used in bacteriophage-based experiments because bacteria grow more rapidly than mammalian cells, thus the evaluation process is faster and more effective. Further improvements in experimental efficiency will accelerate the development of novel antiviral materials.

WebJun 6, 2024 · Therefore, if the screening for active diabodies was enough by only direct MTS assay of culture supernatant, 96-deep well plate would be applied for our semi high-throughput method. dr. yelena shanchuk hoffman estates ilWebApr 14, 2024 · Accurately and rapidly counting the number of maize tassels is critical for maize breeding, management, and monitoring the growth stage of maize plants. With the … comma before so or after in a sentenceWebThe optimized high-throughput shotgun immunoproteomics approach described herein identifies more than five times as many unique antigens as the previously published method, greatly reduces protocol cost and mass spectrometry time per experiment, minimizes both inter- and intra-experimental variability, and ensures each experiment is fully ... dr ye li worcester maWebA high-throughput LC-MS/MS method for simultaneous determination of direct oral anticoagulants in human plasma J Sep Sci. 2024 Apr 4; e2300084. ... In the present study, … dr yellachich comma before thanks toWebJun 5, 2024 · To address these shortcomings, we developed Visual Cell Sorting, a flexible and simple high-throughput method that uses commercial hardware to enable the investigation of cells according to visual phenotype. Visual Cell Sorting is an automated platform that directs a digital micromirror device to mark single live cells that express a … dr yelena levin fax numberWebApr 3, 2024 · A 'high' throughput method is simply a faster method, which allows a greater number of samples to be processed in the same, or less, time. This can be achieved by … comma before that is