Lamina joint inclination assays

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August undefined, 2024

WebBrassinosteroid (BR)-induced rice (Oriza sativa L.) lamina joint (RLJ) inclination and its relationship to indole-3-acetic acid (IAA) and ethylene were investigated using BR … WebAug 1, 2009 · The lamina joint bending assay is established as an indicator to evaluate the effects of plant hormones, herbi- cides and light on rice leaf bending, which is thought to affect crop yields (...

IJMS Free Full-Text The Rice Basic Helix–Loop–Helix 79 …

WebDec 1, 2024 · OsIAA6 interacts with OsARF1 to suppress auxin signaling and to promote cell elongation at the adaxial side of the lamina joint, leading to enlarged leaf inclination. OsBZR1 binds directly to OsIAA6 promoter and OsIAA6 mediates the synergistic effects of BR and auxin on lamina joint development. OsIAA6 may interact with other OsARFs to … WebApr 2, 2024 · Lamina joint of the third leaf from the top of main stem was used for longitudinal section assay. Bar = 50μm. (E) Lamina joint adaxial and abaxial cell lengths. Values represent means ± sd ... Conversely, the reduced leaf inclination and lamina joint cell length phenotypes of the SPX1 overexpressors were only slightly repressed by Pi ... robert purvin md

Molecular actions of two synthetic brassinosteroids, iso-carbaBL …

WebA rice lamina inclination test that is simple and specific for brassinosteroids was used as a micro-quantitative bioassay for brassinolide 1 and its 6-keto congener, castasterone 2, in … WebApr 2, 2024 · We further identified an SPX1-interacting protein, REGULATOR OF LEAF INCLINATION1 (RLI1), which positively regulates leaf inclination by affecting lamina joint cell elongation in rice. The rli1mutants showed reduced leaf inclination and the RLI1overexpressors showed increased leaf inclination. WebJul 20, 2024 · To show that lamina joint inclination of osbul1 is less-sensitive than WT to exogenous brassinolide (BL) treatment in the lamina joint inclination bioassays, we … robert purcell community center cornell

Methyl jasmonate inhibits lamina joint inclination by

Activity of brassinosteroids in the dwarf rice lamina inclination ...

WebJul 3, 2014 · Leaf angle (lamina inclination or lamina joint bending) is the degree of bending between the leaf blade and leaf sheath or the inclination between leaf blade and culm. ... The assays were conducted as described previously (Zhang et al. 2012). Agrobacterium-mediated transfection of N. benthamiana leaves and polyethylene glycol … WebJan 29, 2016 · For the lamina inclination assays, 1 µl of ethanol containing a series of concentrations of BRs, IAA or Brz was spotted onto the tips of the lamina of 5- or 6-day-old seedlings. C-22-hydroxylated and 6-deoxo brassinosteroids were identical to those described by Nakamura et al. (2009). robert putt obituary ravenna ohioWebMay 12, 2024 · Interestingly, ELT1 is preferentially expressed at the lamina joint (Figure 1E) and tiller buds (Figure 1F), and transcribed throughout the lamina joint development (Supplementary information, Figure S5A and S5B), which is consistent with the altered leaf inclination and tiller numbers in mutants. robert purvis

"WebJul 20, 2024 · To show that lamina joint inclination of osbul1 is less-sensitive than WT to exogenous brassinolide (BL) treatment in the lamina joint inclination bioassays, we … " - Lamina joint inclination assays

Lamina joint inclination assays

IJMS Free Full-Text The Rice Basic Helix–Loop–Helix 79 …

WebLamina joint inclination assay in wild type and OsBLR1-transgenic lines. a, c and e The lamina joint inclination assay using different concentration of eBL in blr1-D, BLR1-OE …

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WebJul 3, 2024 · Lamina joint inclination assay. Seeds are sterilized with 2.5% sodium hypochlorite solution, soaked in the dark at 37 °C for 2–3 days, and then germinated in a light incubator under long-day conditions (16L/8D, 28 °C, 70% humidity) for 3 days. ... Lamina inclination assays and expression analysis of BR-related gene between WT … WebLamina joint inclination or leaf angle (the angle between the leaf blade and vertical culm) is a major trait of rice plant architecture. The plant hormone brassinosteroid (BR) is the …

WebMar 18, 2024 · Scanning electron microscopy revealed that the expansion of cell size in the adaxial side of the lamina joint was responsible for the increase in leaf angle in osbhlh079-D. The expression of... WebMar 18, 2024 · The lamina joint, which connects the leaf blade and leaf sheath, is central in controlling leaf angle [ 4 ], as the degree of leaf inclination largely depends on cell proliferation or cell expansion as well as the cell wall composition at the lamina joint.

WebMar 9, 2024 · Lamina joint inclination assays showed that the lamina joint bending angles of the T 1 progenies from the uORF OsBRI1 (−99, 28 aa)#14 were smaller than … WebJan 1, 2024 · Leaf angle is determined by lamina joint inclination and is an important agronomic trait that determines plant architecture, photosynthetic efficiency, and crop yield. Cytokinins (CKs) are phytohormones involved in shaping rice (Oryza sativa L.) architecture, but their role in leaf angle remains unknown.

WebApr 14, 2024 · Furthermore, a BR-induced lamina joint inclination assay (Wada et al., 1984) confirmed that the sensitivity of OsMYB7 overexpressors and the osmyb7-1 mutant are not altered by BR treatments, compared to the WT (Supplementary Figure S6). Taken together, these findings suggest that OsMYB7 is involved in the regulation of leaf angles …

WebMay 12, 2024 · (G) Compared to ZH11, elt1-D or elt1/ELT1-RNAi seedlings display increased or reduced lamina joint inclination of etiolated leaves under 24-eBL treatment. Eight-day-old seedlings were analyzed and ... robert putzWebDec 1, 2015 · The rice lamina joint inclination assay is an extremely sensitive assay for detecting BR activity, based on measuring the angles between the lamina and the … robert putnam making democracy workWebFeb 1, 2013 · Lamina joint inclination assay and morphological analysis in darkness further showed that Fn189 mutant plant was insensitive to exogenous eBL. Through map-based cloning, Fn189 was found to be a novel allelic mutant of the DWARF 61 ( D61) gene, which encodes the putative BRs receptor OsBRI1. robert putnam the upswingWebNational Center for Biotechnology Information robert pye np macon gaWebOct 3, 2024 · In the present study, lamina joint inclination analysis was applied to evaluate the potential interaction between BR and ABA in controlling leaf angle of rice. A number … robert putnam bowling aloneWebFigure 3. BR-induced lamina joint inclination in WT (Hwayoung cultivar) and OsBUL1 knock-out mutant (osbul1) rice. The photo was taken after BL (10-6 M) treatment for 2 … robert q airbus windsorWebNov 29, 2024 · We functionally characterize a novel rice SPOC domain-containing protein leaf inclination 3 (LC3), whose deficiency (lc3 mutant) presents enhanced leaf angle due to the excessive cell elongation at adaxial side of lamina joint, and demonstrate that LC3 controls leaf inclination by regulating auxin signaling through interacting with LIP1, a HIT ... robert q airbus